Techniques in Cytology – Cytospin: Cytospin Cell Resuspension Solution
In this blog series opening article, I discussed how cytospins could be used to separate cancer cells from non-cancer cells1. This cytology method …
In this blog series opening article, I discussed how cytospins could be used to separate cancer cells from non-cancer cells1. This cytology method …
Cytology is a key component in diagnosis and screening of diseases such as cancer. It assesses single cells and clusters of cells from sources such …
Time of Flight Mass Cytometry (CyTOF) is a relatively new multiparametric technology that is far outpacing standard fluorescence-based flow cytometry …
I previously discussed phospho-flow cytometry, a method to study intracellular protein phosphorylation events in peripheral blood mononuclear cells …
In a previous post, I covered the current options for isolating pure cell populations. One immediate question you will have to ask yourself is …
Immunomonitoring of T cell based immune responses spans a wide range of therapeutic applications such as infectious and autoimmune diseases and is …
A healthy and functional immune system requires a delicate balance of pro- and contra-inflammatory signals. Whereas, it is important to induce a …
Changes in circulating cytokine and chemokine levels have been associated with many human diseases, and thus understanding the relationships between …
While many fluorescence-based flow cytometry assays can be run without concern for hitting the exact same fluorescence intensity target values across …
Measuring proliferation of lymphocytes such as T cells isolated from peripheral blood monuclear cells (PBMC) using carboxyfluorescein diacetate …
Innate lymphoid cells (ILCs) are subsets of lymphoid cells that do not rearrange their antigen receptors like T cells and B cells but have other …
Probably the most common way to cryopreserve cells, including human peripheral blood mononuclear cells (PBMC) is using a mixture of 90% serum with …
Dendritic cells (DC) are major antigen-presenting cells consisting of numerous heterogeneous subtypes. In humans, several subtypes of DCs have …
Natural killer (NK) cells represent up to 15% of human peripheral blood mononuclear cells (PBMC), and range from 5-20% of peripheral blood …
Immunologists study many aspects regarding differentiation of T cells and function of T cell lineages. The results and interpretations from these …
Antibody based isolation kits for isolating immune cell populations have become a standard protocol in the toolbox of every immunologist over the …
Negative regulatory CD4 T cells are well characterized and highly studied. However their CD8 counterparts are not well defined, particularly in …
Flow cytometry has been around since the 1950s when Wallace Coulter developed the first flow cytometry device and fluorescence-based flow cytometry …
Antibodies play a significant role in countering foreign threats and ensuring specificity of self/non-self recognition. However, in healthy …
The availability of human peripheral blood mononuclear cells (PBMC) from healthy individuals and from patients with various diseases allows for many …
In Part I, I talked about un-specific binding and Fc-receptor binding. Besides these cases of non-specific binding, there are also other cases of …
CXCR5 is a chemokine receptor expressed by and used to identity human CD4+ follicular helper T cells (TFH). TFH cells, as their name implies, …
The immune privilege of the eye is a widely recognized but frequently oversimplified concept. The notion that the eye possessed unusual immunological …
If you add your antibody, lets say anti-CD3-epsilon antibody, to your cell solution you’d expect that only T cells will be labeled, right? …
Understanding MFI in the context of FACS data The speed, sensitivity and versatility of flow cytometry are things of beauty, but with great power …
Natural Killer (NK) cells are a cytotoxic innate immune lymphocyte cell type. In humans, NK cells comprise up to 15% of peripheral blood …
CD4+ T helper type 1 (TH1) cells are the effector T cell population that governs cell mediated immune responses against intracellular pathogens …
Human peripheral blood mononuclear cells (PBMC) are composed of heterogeneous populations of various immune cell types. CD4+ and CD8+ T cells are …